C. perfringens is a gram-positive, rod-shaped, anaerobic, spore-forming bacterium that is classified into 5 toxinotypes (A, NetF[Please add this to all C.perf on website, as highlighted. ] , B, C, D, and E) according to the 4 major toxins they produce.1 The toxins are common causes of diarrhea, enterocolitis (inflammation of the small and large intestine), enterotoxemia, and colitis (inflammation of the colon) in horses, goats, pigs, and cattle.2 The NetF toxin is one of a series of recently found toxins produced by the spore forming bacteria. Normally, C. perfringens are nonpathogenic and present in the gastrointestinal tract in low numbers. However, antibiotics use, dietary imbalances, and other stressors may cause overgrowth of C. perfringens, leading to GI diseases.3 The disease may occur sporadically or as an outbreak, and clinically affected neonatal foals have high risk of rapid death. Because nonpathogenic serotypes are common, diagnosis is based on identification of toxigenic C. perfringens in fecal samples. PCR detection for toxin-producing genes is recommended.4 Fluxergy’s rapid PCR test is toxin specific and provides reliable detection of the NetF cytotoxin in a point-of-care format.