EQUINE HERPESVIRUS 1

Background

Equine herpesvirus 1 (EHV-1) is an economically important disease in the horse community. It is highly contagious and spreads through direct horse-to-horse contact or indirect contact e.g. contaminated hands, equipment.1,3 Infection manifests into three different syndromes: abortion and early fetal death, respiratory disease, and neurologic disease.1,3 It is a common and burdensome DNA virus in horse populations globally. Moreover, the neurologic form of the disease is particularly troublesome due to associated degrees of paralysis and is seen in approximately 10% of infected horses. Current diagnostic testing requires use of PCR for specific diagnosis as clinical signs of EHV-1 mimic that of equine infl uenza, EHV-4 and other respiratory pathogens.2 EHV-1 imposes further risk due to potentially neuropathic disease thus providing the necessity for species discrimination during diagnosis.2 The Fluxergy EHV-1 assay provides reliable detection of the herpes subtype in deep nasopharyngeal swabs identifying EHV-1 in symptomatic horses or those that have been previously exposed to the virus.3 Further testing is required to confirm whether infection is neuropathic.

Key Benefits

Simple workflow

No sample preparation

Automated data interpretation

Sample to result in 30 minutes

Distinguish EHV-1 from other respiratory pathogens with confidence

Assay Specifications

Sample Type Deep nasopharyngeal swab
Species Equine
Storage Condition Fluxergy Card – Room temperature @ ~23° C
Fluxergy Buffers – Frozen @ -20°C

Kit Contents

  • 6” Rayon swab
  • Fluxergy EHV-1 Buffer 1
  • Fluxergy EHV-1 Buffer 2
  • Fluxergy Card

Customer supplied reagents and supplies

  • Clinical sample for testing

Sample collection method

Collect nasal secretions using a 6” rayon swab. Restrain horse and wear gloves for biosafety. Advance swabs into ventral meatus of right or left nostril. Allow swabs to soak for 5-10 seconds, and then gently rotate swab.

Intended use

The Fluxergy EHV-1 assay is a qualitative test for the rapid detection of equine herpesvirus type 1 in equine respiratory nasopharyngeal swabs. This assay is for Research Use Only (RUO). If positive, further testing is required to confirm neuropathogenic or non-neuropathogenic forms of EHV-1.

Positive PCR Result: indicates that DNA or RNA of the target organism is present in the tested sample.

Negative PCR Result: indicates that DNA or RNA of the target organism was not detected in the tested sample. However, a negative PCR results may also indicate that the number of target organisms is below the limit of detection.

Warnings and Precautions

  • Fluxergy’s EHV-1 assay is for Research Use Only (RUO). It is not intended for diagnostic use.
  • Fluxergy’s EHV-1 assay is compatible only with the Fluxergy Analyzer Beta device.
  • All specimens should be handled as potentially infectious agents and according to universal safety precautions.
  • This Fluxergy EHV-1 assay is compatible only for equine nasal and guttural pouch lavage swabs.
  • Contamination of the sample and kit contents may lead to erroneous results. Use aseptic technique and a clean workspace whenever possible.
  • Store Fluxergy assay kit at recommended storage temperature and conduct assay within specified environment (e.g. temperature and humidity) for optimal performance.
  • Follow appropriate specimen collection, storage and processing for optimal performance.
  • Use Fluxergy supplied swabs, reagents, pipettes and pipette tips to conduct assay for optimal performance.

Detection of Clinical Sample

Clinical sample, specifi cally equine nasopharyngeal swab in PBS positive for EHV-1, was tested using the Fluxergy Analyzer Beta and EHV-1 assay. Results showed amplification and positive agreement with the clinical reference laboratory assay.

References

  1. Rush, B. R. (2016). Equine Herpesvirus Infection. Retrieved from Merck Veterinary Manual.
  2. Pusterla, N., Leutenegger, C. M., Wilson, W. D., Watson, J. L., Ferraro, G. L., & Madigan, J. E. (2005). Equine herpesvirus-4 kinetics in peripheral blood leukocytes and nasopharyngeal secretions in foals using quantiative real-time TaqMan PCR. J Vet Diagn Invest, 17, 578-81.
  3. Patel, J. R., & Haldens, J. (2005). Equine herpesviruses 1 (EHV-1) and 4 (EHV-4)--epidemiology, disease and immunoprophylaxis: a brief review. Veterinary Journal, 1, 14-23.