Equine herpesvirus 4 (EHV-4) is a common DNA virus in horse populations globally. It is most commonly associated with respiratory infection in young horses in higher density populations and is a biosecurity concern due to outbreaks. Symptoms include fever, anorexia, inflammation of lymph nodes and extensive nasal discharge. It is notable that severe economic strain can be seen in the equine industry from EHV-4 due to lost training time, secondary pneumonia and sporadic abortion.1 Further, latent infection and stress-induced shedding is characteristic of the herpesvirus subtypes.1,2 Current diagnostic testing requires use of PCR for specific diagnosis as clinical signs of EHV-4 mimic that of equine influenza, EHV-1 and other respiratory pathogens. EHV-1 imposes further risk due to potentially neuropathic disease thus providing the necessity for species discrimination during diagnosis.2 The Fluxergy EHV-4 assay provides reliable detection of the herpes subtype in deep nasopharyngeal swabs identifying EHV-4 in horses with respiratory symptoms and horses that have been previously exposed to the virus.3

Key Benefits

Simple workflow

No sample preparation

Automated data interpretation

Sample to result in 30 minutes

Detect EHV-4 and distinguish from other respiratory pathogens with confidence

Assay Specifications

Sample Type Deep nasopharyngeal swab
Species Equine
Storage Condition Fluxergy Card – Room temperature @ ~23° C
Fluxergy Buffers – Frozen @ -20°C

Kit Contents

  • 6” Rayon swab
  • Fluxergy EHV-4 Buffer 1
  • Fluxergy EHV-4 Buffer 2
  • Fluxergy Card

Customer supplied reagents and supplies

  • Clinical sample for testing

Sample collection method

Collect nasal secretions using a 6” rayon swab. Restrain horse and wear gloves for biosafety. Advance swabs into ventral meatus of right or left nostril. Allow swabs to soak for 5-10 seconds, and then gently rotate swab.

Intended use

The Fluxergy EHV-4 assay is a qualitative test for the rapid detection of pathogenic equine herpesvirus type 4 in equine respiratory nasopharyngeal swabs. This assay is for Research Use Only (RUO).

Positive PCR Result: indicates that DNA or RNA of the target organism is present in the tested sample.

Negative PCR Result: indicates that DNA or RNA of the target organism was not detected in the tested sample. However, a negative PCR results may also indicate that the number of target organisms is below the limit of detection.

Warnings and Precautions

  • Fluxergy’s EHV-4 assay is for Research Use Only (RUO). It is not intended for diagnostic use.
  • Fluxergy’s EHV-4 assay is compatible only with the Fluxergy Analyzer Beta device.
  • All specimens should be handled as potentially infectious agents and according to universal safety precautions.
  • This Fluxergy EHV-4 assay is compatible only for equine nasal and guttural pouch lavage swabs.
  • Contamination of the sample and kit contents may lead to erroneous results. Use aseptic technique and a clean workspace whenever possible.
  • Store Fluxergy assay kit at recommended storage temperature and conduct assay within specified environment (e.g. temperature and humidity) for optimal performance.
  • Follow appropriate specimen collection, storage and processing for optimal performance.
  • Use Fluxergy supplied swabs, reagents, pipettes and pipette tips to conduct assay for optimal performance.

Detection of Clinical Sample

Clinical sample, specifically equine nasopharyngeal swab in PBS positive for EHV-4, was tested using the Fluxergy Analyzer and EHV-4 assay. Results showed amplification and positive agreement with the clinical reference laboratory assay.


  1. Rush, B. R. (2016). Equine Herpesvirus Infection. Retrieved from Merck Veterinary Manual.
  2. Pusterla, N., Leutenegger, C. M., Wilson, W. D., Watson, J. L., Ferraro, G. L., & Madigan, J. E. (2005). Equine herpesvirus-4 kinetics in peripheral blood leukocytes and nasopharyngeal secretions in foals using quantiative real-time TaqMan PCR. J Vet Diagn Invest, 17, 578-81.
  3. Patel, J. R., & Haldens, J. (2005). Equine herpesviruses 1 (EHV-1) and 4 (EHV-4)--epidemiology, disease and immunoprophylaxis: a brief review. Veterinary Journal, 1, 14-23.