Equine Adenovirus Type 1

Background

Equine adenoviruses (EAdV) are nonenveloped, double-stranded DNA viruses. Two serotypes of equine adenoviruses exist specifically type 1 and type 2. Equine adenovirus type 1 (EAdV1) is widespread globally and causes acute upper respiratory diseases in horses at all ages. It is the causative agent of severe bronchiolitis and pneumonia in immunodeficient Arabian foals. Transmission occurs most commonly by contact with secretions from an infected horse or a contaminated object, also via fecal-oral route. Adenoviruses can be diagnosed by viral culture or serological tests, but they are either time-consuming or non-specific. Fluxergy’s EAdV1 PCR assay provides rapid, sensitive, and specific detection of equine adenoviruses type 1 (EAdV1) at the early stage of infection.

Key Benefits

Simple workflow

No sample preparation

Automated data interpretation

Sample to result in 30 minutes

Early detection can prevent an outbreak

Assay Specifications

Sample Type Deep nasopharyngeal or nasal swab
Species Equine
Storage Condition Fluxergy Card – Room temperature @ ~23° C
Fluxergy Buffers – Frozen @ -20°C

Kit Contents

  • 6” Rayon swab
  • Fluxergy EAdV1 Buffer 1
  • Fluxergy EAdV1 Buffer 2
  • Fluxergy Card

Customer supplied reagents and supplies

  • Clinical sample for testing

Sample collection method

Collect nasal secretions using a 6” rayon swab. Restrain horse and wear gloves for biosafety. Advance swabs into ventral meatus of right or left nostril. Allow swabs to soak for 5-10 seconds, and then gently rotate swab.

Intended use

The Fluxergy EAdV1 assay is a qualitative test for the rapid detection of pathogenic equine adenovirus in equine nasal swabs.

Positive PCR Result: indicates that DNA or RNA of the target organism is present in the tested sample.

Negative PCR Result:  indicates that DNA or RNA of the target organism was not detected in the tested sample. However, a negative PCR results may also indicate that the number of target organisms is below the limit of detection.

Warnings and Precautions

  • Fluxergy’s EAdV1 assay is for Research Use Only (RUO). It is not intended for diagnostic use.
  • Fluxergy’s EAdV1 assay is compatible only with the Fluxergy Analyzer Beta device.
  • All specimens should be handled as potentially infectious agents and according to universal safety precautions.
  • This Fluxergy equine adenovirus assay is compatible only for equine nasal/nasopharyngeal swabs. The sample type must be specified as each buffer is specially crafted for the sample.
  • Contamination of the sample and kit contents may lead to erroneous results. Use aseptic technique and a clean workspace whenever possible.
  • Store Fluxergy assay kit at recommended storage temperature and conduct assay within specified environment (e.g. temperature and humidity) for optimal performance.
  • Follow appropriate specimen collection, storage and processing for optimal performance.
  • Use Fluxergy supplied swabs, reagents, pipettes and pipette tips to conduct assay for optimal performance.

Detection of Clinical Sample

Clinical sample detection. Clinical sample, specifically equine nasal epithelial cell culture positive for equine adenovirus type 1, was tested using the Fluxergy Analyzer Beta and equine adenovirus assay. Results showed amplification and positive agreement with the clinical reference laboratory assay.

References

  1. Rowe, Wallace P., et al. "Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture." Proceedings of the Society for Experimental Biology and Medicine 84.3 (1953): 570-573.
  2. Fenner, Frank J.; Gibbs, E. Paul J.; Murphy, Frederick A.; Rott, Rudolph; Studdert, Michael J.; White, David O. (1993). Veterinary Virology (2nd ed.). Academic Press, Inc. ISBN 0-12-253056-X
  3. Moorthy, A. R. S., and P. B. Spradbrow. "Adenoviral infection of Arab foals with respiratory tract disease." Zoonoses and Public Health 25.6 (1978): 469-477.
  4. Snyder, J. (2006) “The Equine Manual” Elsevier Health Sciences