Equine adenoviruses (EAdV) are nonenveloped, double-stranded DNA viruses. Two serotypes of equine adenoviruses exist specifically type 1 and type 2. Equine adenovirus type 1 (EAdV1) is widespread globally and causes acute upper respiratory diseases in horses at all ages. It is the causative agent of severe bronchiolitis and pneumonia in immunodeficient Arabian foals. Transmission occurs most commonly by contact with secretions from an infected horse or a contaminated object, also via fecal-oral route. Adenoviruses can be diagnosed by viral culture or serological tests, but they are either time-consuming or non-specific. Fluxergy’s EAdV1 PCR assay provides rapid, sensitive, and specific detection of equine adenoviruses type 1 (EAdV1) at the early stage of infection.