Equine influenza virus

Background

Equine influenza virus (EIV) is a highly contagious respiratory disease that is endemic to horses in the US and around the world providing economic morbidity in countries with substantial breeding and racing efforts.1 Significant factors influencing susceptibility are age, common effecting horses 1-5 years of age, and exposure with higher incidence seen in horses living in high density populations.1,2 The virus spreads rapidly in aerosolized droplets dispersed by coughing from infected horses during the 1–3 day incubation period. Although horses can be vaccinated, antigenic drift is an issue as immunity and protection may only last for a few years.3 Symptoms include fever, cough, nasal discharge, and secondary bacterial complications.1,2 H3N8 is the Influenza Type A strain associated with current cases of EIV.1,2,3 The Fluxergy EIV Assay specifically detects the H3N8 strain via PCR of deep nasopharyngeal swab providing immediate turnaround times and accuracy in virus identification.

Key Benefits

Simple workflow

No sample preparation

Automated data interpretation

Sample to result in 30 minutes

Ensure biosafety with reliable EIV detection

Assay Specifications

Sample Type Deep nasopharyngeal swab
Species Equine
Storage Condition Fluxergy Card – Room temperature @ ~23° C
Fluxergy Buffers – Frozen @ -20°C

Kit Contents

  • 6” Rayon swab
  • Fluxergy EIV Buffer 1
  • Fluxergy EIV Buffer 2
  • Fluxergy Card

Customer supplied reagents and supplies

  • Clinical sample for testing

Sample collection method

Collect nasal secretions using a 6” rayon swab. Restrain horse and wear gloves for biosafety. Advance swabs into ventral meatus of right or left nostril. Allow swabs to soak for 5-10 seconds, and then gently rotate swab. Store sample at 4°C if not testing immediately.

Intended use

The Fluxergy EIV assay is a qualitative test for the rapid detection of pathogenic EIV in equine respiratory nasopharyngeal swabs. This assay is for Research Use Only (RUO).

Positive PCR Result: indicates that DNA or RNA of the target organism is present in the tested sample.

Negative PCR Result: indicates that DNA or RNA of the target organism was not detected in the tested sample. However, a negative PCR results may also indicate that the number of target organisms is below the limit of detection.

Warnings and Precautions

  • Fluxergy’s EIV assay is for Research Use Only (RUO). It is not intended for diagnostic use.
  • Fluxergy’s EIV assay is compatible only with the Fluxergy Analyzer Beta device.
  • All specimens should be handled as potentially infectious agents and according to universal safety precautions.
  • This Fluxergy EIV assay is compatible only for equine nasal and guttural pouch lavage swabs.
  • Contamination of the sample and kit contents may lead to erroneous results. Use aseptic technique and a clean workspace whenever possible.
  • Store Fluxergy assay kit at recommended storage temperature and conduct assay within specified environment (e.g. temperature and humidity) for optimal performance.
  • Follow appropriate specimen collection, storage and processing for optimal performance.
  • Use Fluxergy supplied swabs, reagents, pipettes and pipette tips to conduct assay for optimal performance.

Detection of Clinical Sample

Clinical sample detection. PCR was conducted using the Fluxergy Analyzer Beta with clinical EIV positive deep nasal swab solution. Positive result agreement was seen with the Fluxergy and clinical reference lab assays.

References

  1. Rush, B. R. (2016). Equine Influenza. Retrieved from Merck Veterinary Manual.
  2. AAEP. (2017). Equine Influenza. American Association of Equine Practitioners.
  3. Pusterla, N., Kass, P., Mapes, S., Wademan, C., Akana, N., Barnett, C., . . . Vaala, W. (2015). Voluntary Surveillance Program for Equine Influenza Virus in the United States from 2010 to 2013. Journal of Veterinary Internal Medicine, 29, 417-22.