Equine Rotavirus

Background

Rotaviruses are non-enveloped, double-stranded RNA virus which act as causative agents of acute gastroenteritis in various mammalian species.1 Specifically, equine rotavirus is a major cause of dehydrating diarrhea in foals.2 The virus is mainly transmitted via fecal-oral route, also by contact with contaminated surfaces and objects. It is highly contagious and resistant to bleach. Thus, rotaviral diarrhea mostly occurs as outbreaks in horse breeding farms. Rotavirus can be detected by electron microscopy or ELISA, but both are either expensive or non-specific.3 Fluxergy’s point-of-care PCR test provides rapid, sensitive, and specific detection of equine rotavirus at the early stage of infections.

Key Benefits

Simple workflow

No sample preparation

Automated data interpretation

Sample to result in 30 minutes

Assay Specifications

Sample Type Rectal swab
Species Equine
Storage Condition Fluxergy Card – Room temperature @ ~23° C
Fluxergy Buffers – Frozen @ -20°C

Kit Contents

  • 6” Rayon swab
  • Fluxergy Equine Rotavirus Buffer 1
  • Fluxergy Equine Rotavirus Buffer 2
  • Fluxergy Card

Customer supplied reagents and supplies

  • Clinical sample for testing

Sample collection method

For, rectal swab. It is recommended that the head of the swab is fully coated with sample material. Fully submerge the head of the swab into the rectum for 5 seconds. Place mucus coated swab into the swab tube. The opposite end of the tube serves as a tight-fitting cap. Place sample at 4°C if not testing immediately.

Intended use

The Fluxergy equine rotavirus assay is a qualitative test for the rapid detection of pathogenic equine rotavirus in equine rectal swabs.  

Positive PCR Result: indicates that DNA or RNA of the target organism is present in the tested sample.

Negative PCR Result:   indicates that DNA or RNA of the target organism was not detected in the tested sample. However, a negative PCR results may also indicate that the number of target organisms is below the limit of detection.

Warnings and Precautions

  • Fluxergy’s equine rotavirus assay is for Research Use Only (RUO). It is not intended for diagnostic use.
  • Fluxergy’s equine rotavirus assay is compatible only with the Fluxergy Analyzer Beta device.
  • All specimens should be handled as potentially infectious agents and according to universal safety precautions.
  • This Fluxergy equine rotavirus assay is compatible only for equine rectal swab. The sample type must be specified as each buffer is specially crafted for the sample.
  • Contamination of the sample and kit contents may lead to erroneous results. Use aseptic technique and a clean workspace whenever possible.
  • Store Fluxergy assay kit at recommended storage temperature and conduct assay within specified environment (e.g. temperature and humidity) for optimal performance.
  • Follow appropriate specimen collection, storage and processing for optimal performance.
  • Use Fluxergy supplied swabs, reagents, pipettes and pipette tips to conduct assay for optimal performance.

Detection of Clinical Sample

Clinical sample detection. Clinical sample, specifically equine fecal swab positive for equine rotavirus, was tested using the Fluxergy Analyzer Beta and equine rotavirus assay. Results showed amplification and positive agreement with the clinical reference laboratory assay.

References

  1. Hoshino, Y. A. S. U. T. A. K. A., et al. "Isolation and characterization of an equine rotavirus." Journal of clinical microbiology 18.3 (1983): 585-591.
  2. Matthijnssens, Jelle, et al. "Complete molecular genome analyses of equine rotavirus A strains from different continents reveal several novel genotypes and a largely conserved genotype constellation." Journal of general virology 93.4 (2012): 866-875.
  3. Sellon, Debra C., and Maureen Long. Equine Infectious Diseases E-Book. Elsevier Health Sciences, 2013.