lawsonia intracellularis

Background

Lawsonia intracellularis is an intracellular, gram-negative, bacteria that causes Equine Proliferative Enteropathy (EPE) worldwide, and can effect foals, pigs, rodents, and ratites. This disease in foals is also called “Lawsonia” and can survive in the environment for 2 weeks. Transmission may occur by fecal to oral route. Infective signs include: proliferative enteropa-thy, diarrhea, rapid weight loss, colic, lethargy, and subcutaneous edema in weaning foals. Foals 4-6 months are commonly infected, but L. intracellularis may infect foals under a year old. Infected foals may obtain a secondary infection in the gastro intestinal tract, skin, or respiratory tract. Diagnosis of L. intracellularis can be confirmed using Polymerase Chain Reaction (PCR), immunohistochemistry, serology, and ELISA. However, it is recommended that PCR and serology be ordered. Treatment of L. intracellularis with oxytetracycline, followed by doxycycline has been previously effective. This Fluxergy L. intracellularis PCR assay has been designed to target a conserved region of this bacteria and allow for rapid detection of the pathogen within close proximity of the patient.

Key Benefits

Detect infection in foals

Automated data interpretation

Simple workflow

Sample to result in 30 minutes

No sample preparation

Assay Specifications

Sample Type Feces, fecal swab
Species Equine
Storage Condition Fluxergy Card – Room temperature @ ~23°C
Fluxergy Buffers – Frozen @ 4°C

Kit Contents

  • 6” Rayon swab
  • Fluxergy L. intracellularis Buffer 1
  • Fluxergy L. intracellularis Buffer 2
  • Fluxergy Card

Customer supplied reagents and supplies

  • Clinical sample for testing

Sample collection method

Fecal swab. It is recommended that the head of the swab is fully coated with sample material. For optimal performance, collect feces to be tested in a sterile 2 oz specimen cup. Fully submerge the head of the swab into the sample for 5 seconds. Place stool coated swab into the swab tube. The opposite end of the tube serves as a tight-fitting cap. Store sample in refrigerator or 4°C if not testing immediately after sample collection.

Intended use

The Fluxergy L. intracellularis assay is a qualitative test for the rapid detection of pathogenic L. intracellularis in equine fecal samples.

Positive PCR Result: indicates that DNA or RNA of the target organism is present in the tested sample.

Negative PCR Result: indicates that DNA or RNA of the target organism was not detected in the tested sample. However, a negative PCR results may also indicate that the number of target organisms is below the limit of detection.

Warnings and Precautions

  • Fluxergy’s L. intracellularis assay is for Research Use Only (RUO). It is not intended for diagnostic use.
  • Fluxergy’s L. intracellularis assay is compatible only with the Fluxergy Analyzer Beta device.
  • All specimens should be handled as potentially infectious agents and according to universal safety precautions.
  • This Fluxergy L. intracellularis assay is compatible only for equine fecal swabs.
  • Contamination of the sample and kit contents may lead to erroneous results. Use aseptic technique and a clean workspace whenever possible.
  • Store Fluxergy assay kit at recommended storage temperature and conduct assay within specified environment (e.g. temperature and humidity) for optimal performance
  • Follow appropriate specimen collection, storage and processing for optimal performance.
  • Use Fluxergy supplied swabs, reagents, pipettes and pipette tips to conduct assay for optimal performance

Detection of Clinical Sample

Clinical sample detection. PCR was conducted using the Fluxergy Analyzer Beta with clinical L. intracellularis positive equine feces. Positive result agreement was seen with the Fluxergy and clinical reference lab assays.

References

  1. Michael L Frazer et al.; How to Diagnose and Treat Lawsonia intracellularis, AAEP Proceedings, Volume 53, 200, Pages 236–239.
  2. Stewart, A. J. (2016). Equine Proliferative Enteropathy. Retrieved from Merck Veterinary Manual.