STREPTOCOCCUS EQUI SUBSP. EQUI

Background

Streptococcus equi equi is a gram-positive bacteria that induces infection and contagious disease in the upper respiratory tract of horses, donkeys and mules. Symptoms of clinical disease, termed strangles, include bacterial colonization of the patient’s tonsils and pharynx resulting in abscessation of the lymph nodes under and behind the jaw. It is essential to isolate any horse with signs of strangles to prevent population outbreaks. The incubation period of strangles is up to 2 weeks and symptoms will be evident within 1–2 days of the onset fever. Current diagnostic testing includes detection of shedding via bacterial culture and more sensitively with molecular PCR confirmation.1 The Fluxergy S. equi equi assay detects a conserved region of the S. equi equi genome that is highly unique to the specifi c streptococcus subtype. Use the Fluxergy system to diagnose strangles with speed and sensitivity and enhance biosecurity within your clinic.

Key Benefits

Simple workflow

No sample preparation

Automated data interpretation

Sample to result in 30 minutes

Specific diagnosis of strangles

Isolate contagious horses immediately

Assay Specifications

Sample Type Swab of nasal or guttural pouch washes in buffered saline
Species Equine
Storage Condition Fluxergy Card – Room temperature @ ~23° C
Fluxergy Buffers – Frozen @ -20°C

Kit Contents

  • 6” Rayon swab
  • Fluxergy S equi equi Buffer 1
  • Fluxergy S equi equi Buffer 2
  • Fluxergy Card

Customer supplied reagents and supplies

  • Clinical sample for testing

Sample collection method

For nasal swab, keep swab in contact with the mucosa for a minimum of 20 seconds to ensure good contact. For guttural pouch lavage, collect saline wash in a sterile 2 oz. specimen cup. Submerge provided swab in specimen cup for a minimum of 10 seconds for full coverage of the swab. Place sample coated swab into the swab tube. The opposite end of the tube serves as tight-fitting cap. Collect in sterile container. Store sample in refrigerator or at 4°C if not testing immediately after sample collection.

Intended use

The Fluxergy S equi equi assay is a qualitative test for the rapid detection of pathogenic Streptococcus equi equi in equine nasopharyngeal and guttural pouch lavage swabs. This assay is for Research Use Only (RUO).

Positive PCR Result: indicates that DNA or RNA of the target organism is present in the tested sample.

Negative PCR Result: indicates that DNA or RNA of the target organism was not detected in the tested sample. However, a negative PCR results may also indicate that the number of target organisms is below the limit of detection.

Warnings and Precautions

  • Fluxergy’s S equi equi assay is for Research Use Only (RUO). It is not intended for diagnostic use.
  • Fluxergy’s S equi equi assay is compatible only with the Fluxergy Analyzer device.
  • All specimens should be handled as potentially infectious agents and according to universal safety precautions.
  • This Fluxergy S equi equi assay is compatible only for equine nasal and guttural pouch lavage swabs.
  • Contamination of the sample and kit contents may lead to erroneous results. Use aseptic technique and a clean workspace whenever possible.
  • Store Fluxergy assay kit at recommended storage temperature and conduct assay within specified environment (e.g. temperature and humidity) for optimal performance.
  • Follow appropriate specimen collection, storage and processing for optimal performance.
  • Use Fluxergy supplied swabs, reagents, pipettes and pipette tips to conduct assay for optimal performance.

Clinical Performance

Fluxergy Streptococcus equi subsp. equi Assay versus NAAT

26 streptococcus equi subsp. equi positive nasal swab samples were tested using the Fluxergy Analyzer Beta streptococcus equi subsp. equi Assay. 15 streptococcus equi subsp. equi negative nasal swab samples were also tested. Clinical nasal swabs were inverted into 1ml of PBS solution and frozen at 4°C prior to testing. Samples were acquired from the UC Davis Real-time PCR Research and Diagnostics Core Facility and categorized by a standard of care, qualitative PCR test.

Fluxergy Result Standard of Care Testing
Detected 26 0 26
Not Detected 0 15 15
Total 26 15 41
Positive Agreement (26/26) Negative Agreement (15/15)

Cross Reactivity

A common concern when testing for streptococcus equi subsp. equi is cross reactivity with other bacteria, specifically of the same genus. PCR positive streptococcus equi subsp. zooepidemicus nasal swab samples were tested for potential cross reactivity with the Fluxergy assay. Samples were acquired from the UC Davis Real-time PCR Research and Diagnostics Core Facility and tested using a standard of care, qualitative PCR test. All samples tested negative for the presence of streptococcus equi subsp. equi. Clinical nasal swabs were inverted into 1ml of PBS solution and frozen at 4°C prior to testing.

Organism Tested Concentration Fluxergy Streptococcus equi subsp. equi Results:% Detection (#Detected/#Tested)
Streptococcus equi subsp. zooepidemicus Unknown, PCR positive 0.0% (0/5)

Detection of Clinical Sample

Clinical sample, specifically equine guttural pouch lavage positive for S. equi equi, was tested using the Fluxergy Analyzer and S. equi equi assay. Results showed amplification and positive agreement with the clinical reference laboratory assay.

References

  1. Rush, B. R. (2016). Strangles in Horses. Retrieved from Merck Veterinary Manual.