Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a gram-positive coccoid bacterium found in the upper respiratory tract of horses, pigs, and humans. In horses, S. zooepidemicus is a commensal of nasopharynx and an opportunistic pathogen causing respiratory disease. Although, it is mainly associated with pneumonia, pleuropneumonia, and mastitis in horses, it has the potential to infect humans and causes more severe infections. Streptococcus equi subsp. equi, another subspecies of Streptococcus equi, is the causative agent of strangles. The similarity between the DNA of the two close-related subspecies is over 92%, making the molecular detection and differentiation of each subspecies more challenging. Fluxergy’s S. zooepidemicus rapid detection test is designed to have high specificity to the S. zooepidemicus genome to minimize the chance of false-positive results and provides reliable detection in a point-of-use format.