When transferring fluids between steps of our test, proper pipetting techniques are critical to limiting user error. Here are some guidelines to help you with that.
- Use Pipette tips with a filtered aerosol barrier.
This helps to prevent carryover contamination.
- Only use tips once.
- Pipette slowly and carefully
- Ensure that you have pipetted the full volume, whether that is 14 µL or 130 µL.
Limited sample or reaction mixture volumes can negatively affect your test result.
- Avoid pipetting air bubbles
You can check for air bubbles by visually observing your pipette tip and Sample/Reaction Mixture vials. Air bubbles can adversely affect the performance of microfluidics.