Environmental Salmonella -PCR Fluxergy Test Kit
A simple and cost-effective, accurate test to identify Salmonella within the veterinary hospital environment, helping ensure biosecurity protocols are sufficient.
- Improve biosecurity and boost client confidence to mitigate risk of hospital acquired infection (HAI).
- Confirm disinfection protocol is effective and increase speed of stall turnover.
- Deliver results in < 1 hour after 16-24 hour incubation. Reference laboratory qPCR testing can take up to 3-5 days.
- Simple 10-minute test protocol following aerobic incubation allows the team to set up the test and move on with their day.
Clinically evaluated to ensure performance
Analytical Reactivity
This study is designed to assess the performance of Fluxergy PCR Salmonella Assay with Salmonella serotypes. Specific serotypes were selected due to their clinical relevance. A total of 20 serotypes were tested on the Fluxergy platform.
Cross Reactivity
Cross Reactivity of the Fluxergy Environmental Salmonella Test Kit was evaluated by wet-lab testing using the Fluxergy Analyzer. A panel of 23 microorganisms were tested at a concentration of 1X10^5 copies/mL. All organisms tested negative in triplicate, indicating no cross-over with non-Salmonella targets tested.
Limit of Detection (LOD)
Salmonella spp. was serially diluted in negative pooled environmental swab matrix down to 1CFU/mL. 1mL of 1CFU/mL was spiked into BD BBL™ Mycoflask™ Selenite Cystine Broth Prepared Media (BD 297711). Culture was incubated (GBiosciences Incubator Shaker overnight at 37 C for 16 hours.) The LOD of 1 CFU/mL was confirmed by detecting > 95% of 20 replicates tested.
16 hours for enrichment, ~3 min from reagent thaw
InvA and ttrC
(10.31 inches x 5.16 inches x 10.16 inches)
Save cost with faster stall turnover
On-site testing allows for quick answers when dealing with suspect cases:
- ICU stalls (Prior to patient admit )
- Surgical suites (Post-colic surgery)
- Horse trailers (Post-travel for stress-induced shedding)
- Routine screening of probable practice locations
- Clinics or referral hospitals
- Ambulatory practices
- Veterinary teaching hospitals
FAQs
Salmonella is a leading cause of enterocolitis in susceptible horses. Clinical cases range from asymptomatic to acute and severe diarrhea to death. Further, it is possible for disease outbreaks and population epidemics to occur depending on the organism, host and degree of exposure. It is thus recommended that PCR be used for rapid and specific pathogen detection to maintain biosecurity.
Salmonella can be isolated from a mat located in the colic surgery room. 18 of the 33 cases in this outbreak had surgery, and it is possible that some cases were infected during surgery. In other outbreaks in teaching hospitals, Salmonella has been isolated from a range of sites, including horse feeders and waterers, floor drains, stalls and stall mats, operating tables, recovery room mats and office floors.
The risk of Salmonella shedding more than doubled in patients with abdominal surgery versus non-surgical patients. Patients who underwent abdominal surgery were also eight times more likely to contract a hospital-acquired Salmonella infection.
To reduce fomite risk of Salmonella contamination, test nasogastric tubing and buckets, manure pitchforks, and ambulatory vehicle floor mats, tires and steering wheels, and tractor tires. Salmonella has been frequently isolated from reflux samples, so the risk of Salmonella environmental contamination and hospital-acquired infections is increased. Improved environmental monitoring allows for earlier detection and isolation, reducing environmental contamination.
Adult horses that had been treated with antimicrobial drugs prior to hospitalization were 3.09 times as likely to be shedding Salmonella organisms as were adult horses that had not been treated with antimicrobial drugs prior to hospitalization.
- Ward, M. P., Brady, T. H., Couëtil, L. L., Liljebjelke, K., Maurer, J. J., & Ching, C. W. (2005). Investigation and control of an outbreak of salmonellosis caused by multidrug-resistant Salmonella typhimurium in a population of hospitalized horses. Veterinary Microbiology, 107(3–4), 233–240. https://doi.org/10.1016/J.VETMIC.2005.01.019
- Ernst, N. S., Hernandez, J. A., MacKay, R. J., Brown, M. P., Gaskin, J. M., Nguyen, A. D., Giguere, S., Colahan, P. T., Troedsson, M. R., Haines, G. R., Addison, I. R., & Miller, B. J. (2004). Risk factors associated with fecal Salmonella shedding among hospitalized horses with signs of gastrointestinal tract disease. Journal of the American Veterinary Medical Association, 225(2), 275–281. https://doi.org/10.2460/JAVMA.2004.225.275
- Ekiri, A. B., MacKay, R. J., Gaskin, J. M., Freeman, D. E., House, A. M., Giguère, S., Troedsson, M. R., Schuman, C. D., Von Chamier, M. M., Henry, K. M., & Hernandez, J. A. (2009). Epidemiologic analysis of nosocomial Salmonella infections in hospitalized horses. Journal of the American Veterinary Medical Association, 234(1), 108–119. https://doi.org/10.2460/JAVMA.234.1.10
- Rothers, K. L., Hackett, E. S., Mason, G. L., & Nelson, B. B. (2020). Atypical Salmonellosis in a Horse: Implications for Hospital Safety. Case Reports in Veterinary Medicine, 2020. https://doi.org/10.1155/2020/7062408
- Dallap Schaer, B. L., Aceto, H., Caruso, M. A., & Brace, M. A. (2012). Identification of Predictors of Salmonella Shedding in Adult Horses Presented for Acute Colic. Journal of Veterinary Internal Medicine, 26(5), 1177–1185. https://doi.org/10.1111/J.1939-1676.2012.00984.X
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Testimonials
"I have conducted research on the Fluxergy system for the past 3 years. Having the capability to have a patient-side, reliable, sensitive, and cost-effective molecular detection instrument can be a tremendous improvement in the diagnostic field. Fluxergy’s advanced technology can potentially be used on clinical and subclinical horses in order to allow for proper treatment and timely biosecurity. Fluxergy’s novel platform can turn a 1 to 3-day turnaround time into a 1-hour processing time."
Dr. Nicola Pusterla
UC Davis School of Veterinary Medicine
"Fluxergy is at the forefront of diagnostic capabilities with PCR. The PCR run-time is quick and easy. As we improve our care through newer technologies and diagnostics, such as Fluxergy, we better educate our clients on what is causing the problem."
Paul Wan
Veterinarian Practitioner Owner at SoCal Equine Hospital
"The decision to establish a lab on-site was made easy given the technology and support we received from Fluxergy. Most importantly, it gave us an important new tool for our biosecurity program and greatly enhanced our ability to assess the risk created by a horse with a fever. Identifying potential sick horses is the most important priority in preventing another outbreak, and with Fluxergy's technology, we are better prepared."
Steve Hankin
Desert Horse Park International