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Fluxergy has the honor of being included in three abstracts being shown at the American Association of Veterinary Laboratory Diagnostics Conference (AAVLD) early October.
Validation of a point-of-care PCR assay for the detection of equine herpesvirus 1 in nasal secretions and whole blood
Timely and cost-effective detection of equine herpesvirus 1 (EHV-1) is crucial for preventing and managing disease outbreaks. The recent introduction of microfluidic card technology allows for on-site rapid testing within one hour, including sample collection, processing, and PCR analysis. Given the high morbidity associated with clinical EHV-1, accurate detection is essential for identifying infectious horses during early disease states but also for effective outbreak management.
Purpose: We aimed to evaluate a point-of-care (POC) PCR assay for the detection of EHV-1 in nasal secretions and whole blood and compare the results with the gold standard of quantitative PCR (qPCR).
Investigation of large volume samples for the detection of Streptococcus equi ssp. equi using a novel point-of-care PCR assay
Strangles is a highly contagious, upper respiratory tract infection that affects horses and other equines caused by Streptococcus equi subspecies equi (S. equi). Quantitative PCR (qPCR) assays for the detection of S. equi in nasal swabs, and large volume samples such as nasopharyngeal washes (NPW) and guttural pouch lavages (GPL), are considered the molecular ‘gold standard’ for diagnosis of strangles. A novel point-of-care (POC) PCR assay has recently been validated for rostral nasal secretions, however, the assay has yet to be validated using large volume samples.
Purpose: To evaluate a POC PCR assay for the detection of S. equi in large volume samples collected from horses (clinical and subclinical) and compare it to the gold standard of qPCR.
Performance of a point-of-care PCR assay for the detection of Salmonella spp. in pooled environmental samples
A timely and cost-effective detection of Salmonella spp. in environmental samples is important from a biosecurity standpoint. The recent introduction of microfluidic card technology for the detection of Salmonella spp. in feces and environmental samples allows testing of individual samples in less than 24 hours, including sample collection, enrichment step and PCR analysis. Because of the low but impactful detection rate of Salmonella spp. in equine hospitals, contemporary protocols presently aim at pooling environmental samples after sample enrichment.
Purpose: To evaluate a novel point-of-care (POC) PCR assay for the detection of Salmonella spp. in pooled environmental samples collected at a Veterinary Medical Teaching Hospital.
